Linkage Analysis in an Octasaccharide from Prosopis juliflora by Methylation,Periodate Oxidation and NMR studies

Prosopis juliflora (Sw.) DC. commonly known as mesquite, vilayti babul, vilaytikikar belongs to family leguminosae sub family (mimosoidae). P. juliflora is often quoted as native to the Caribbean where it is found often in coastal areas.It’s a thorny ever-green to semi-evergreen fast growing tree capable of growing in arid and semi-arid regions and attaining heights up to 10m. A polysaccharide was isolated from Prosopis juliflora seed and purified. Purified juliflora polysaccharide has been partially hydrolysed to get structural oligosaccharides. An oligosaccharide has been isolated from the hydrolysed product of Prosopis juliflora seed polysaccharide. On complete hydrolysis it furnished two monosugars, galactose and mannose in the molar ratio of 3:5. Methylation,H NMR,C NMR studies and periodate oxidation indicated the presence of (1→4) and (1→6) linkages among structural monosaccharide units.